The　simultaneous　regeneration　of　articular　cartilage　and　subchondral　bone　is　a　major　challenge.　Bioinspired　scaffolds　with　distinct　regions　resembling　stratified　anatomical　architecture　provide　a　potential　strategy　for　osteochondral　defect　repair.　Here,　we　report　the　development　of　an　injectable　and　bilayered　hydrogel　scaffold　with　a　strong　interface　binding　force.　In　this　bilayer　hydrogel,　composed　of　carbonyl　hydrazide　grafted　collagen　(COL-CDH)　and　oxidized　chondroitin　sulfate　(OCS),　which　are　derivatives　of　osteochondral　tissue　components,　in　combination　with　poly　(ethylene　glycol)　diacrylate　(PEGDA),　functions　as　a　cartilage　layer;　while　zinc-doped　hydroxyapatite　acts　as　a　subchondral　bone　layer　that　is　based　on　the　cartilage　layer.　The　strong　interface　between　the　two　layers　involves　dynamic　amide　bonds　formed　between　COL-CDH　and　OCS,　and　permanent　C–C　bonds　formed　by　PEGDA　radical　reactions.　This　bilayer　hydrogel　can　be　used　to　inoculate　adipose　mesenchymal　stem　cells　which　can　then　differentiate　into　chondrocytes　and　osteoblasts,　secreting　glycosaminoglycan,　and　promoting　calcium　deposition.　This　accelerates　the　regeneration　of　cartilage　and　subchondral　bone.　Micro-CT　and　tissue　staining　revealed　an　increase　in　the　amount　of　bone　present　in　new　subchondral　bone,　and　new　tissues　with　a　structure　similar　to　normal　cartilage.　This　study　therefore　demonstrates　that　injectable　bilayer　hydrogels　are　a　promising　scaffold　for　repairing　osteochondral　defects.　©　2023　Elsevier　B.V.