Murals　are　a　significant　cultural　heritage　of　humanity,　and　one　of　the　conservation　studies　is　to　control　the　growth　of　microorganisms.　General　biocide　agents　can　be　used　to　preserve　murals　while　also　providing　new　organic　carbon　sources　and　increasing　environmental　pollution.　In　recent　years,　radiation　technology　has　shown　promising　prospects　for　use　in　heritage　protection.　Five　microorganisms　often　found　in　murals　were　irradiated　with　an　electron　beam　in　this　study,　and　six　mineral　pigments　were　tested　for　color　change,　Raman　spectra　and　pigment　layer　cohesion　after　irradiation.　The　result　showed　that　irradiation　at　20　kGy　can　basically　eliminate　Pseudomonas　citronellolis,　Bacillus　sporothermodurans,　Streptomyces　vinaceus,　and　Streptomyces　griseolus　from　the　culture　medium,　but　only　inhibited　the　growth　of　Penicillium　flavigenum.　Lead　white　pigment　showed　a　color　difference　of　5.56　(　increment　E*(97))　after　irradiation,　but　lead　tetroxide,　azurite,　malachite,　ferrous　oxide,　and　cinnabar　showed　no　visible　changes.　The　Raman　spectra　of　the　irradiated　and　unirradiated　samples　were　basically　the　same.　E-beam　radiation　did　not　affect　the　surface　cohesion　of　the　pigment　layer.　This　preliminary　work　shows　the　potential　of　electron-beam　technology　in　mural　protection　and　provides　basic　research　and　relevant　experience　for　the　subsequent　in　situ　mural　protection　work.