Foodborne　pathogens　like　Listeria　monocytogenes　can　cause　various　illnesses　and　pose　a　serious　threat　to　public　health.　They　produce　species-specific　microbial　volatile　organic　compounds,　i.e.,　the　biomarkers,　making　it　possible　to　indirectly　measure　microbial　contamination　in　foodstuff.　Herein,　highly　ordered　mesoporous　tungsten　oxides　with　high　surface　areas　and　tunable　pores　have　been　synthesized　and　used　as　sensing　materials　to　achieve　an　exceptionally　sensitive　and　selective　detection　of　trace　Listeria　monocytogenes.　The　mesoporous　WO3-based　chemiresistive　sensors　exhibit　a　rapid　response,　superior　sensitivity,　and　highly　selective　detection　of　3-hydroxy-2-butanone.　The　chemical　mechanism　study　reveals　that　acetic　acid　is　the　main　product　generated　by　the　surface　catalytic　reaction　of　the　biomarker　molecule　over　mesoporous　WO3.　Furthermore,　by　using　the　mesoporous　WO3-based　sensors,　a　rapid　bacteria　detection　was　achieved,　with　a　high　sensitivity,　a　linear　relationship　in　a　broad　range,　and　a　high　specificity　for　Listeria　monocytogenes.　Such　a　good　gas　sensing　performance　foresees　the　great　potential　application　of　mesoporous　WO3-based　sensors　for　fast　and　effective　detection　of　microbial　contamination　for　the　safety　of　food,　water　safety　and　public　health.