Extractive　electrospray　ionization　of　1-hydroxypyrene　(1-OHP)　is　achieved　by　using　non-polar　solvents　(e.g.,　benzene,　toluene,　xylene,　pentane　and　hexane)　as　the　primary　ESI　solvent.　The　extraction　of　1-OHP　from　various　polar　sample　solutions　by　a　non-polar　primary　ESI　solvent　is　identified　and　1-OHP　ionization　efficiency　strongly　depends　on　the　sample　solution.