Nanotechnology　has　gained　massive　applications　in　the　fields　of　biology　and　pharmacology.　Recently,　more　research　attention　has　been　paid　to　explore　the　interaction　between　nanoparticles　(NPs)　and　cells,　in　particular,　the　entry　of　NPs　into　cells.　Herein,　we　focus　on　the　internalization　of　the　FITC-labeled　silica-coated　titania　NPs　(FITC-TiO2@SiO2　NPs)　in　neural　stem　cells　(NSCs)　and　their　subsequent　translocation.　Various　microscopic　techniques,　such　as　confocal-laser-scanning　microscopy　(CFLSM),　transmission　electron　microscopy　(TEM)　and　atomic　force　microscopy　(AFM)　were　used　to　track　the　time-dependent　pathway　of　NPs　in　cells.　It　was　found　that　NPs　traversed　cell　membrane　and　localized　around　the　cell　nucleus.　Most　NPs　congregated　in　lysosomes　and　were　transformed　by　lysosomes　after　48　h　co-incubation.