A　static　in　vitro　digestion　model　simulating　human　gastrointestinal　tract　was　used　to　investigate　digestion　mechanism　of　polychlorinated　biphenyls　(PCBs)　in　food　during　digestion.　The　results　showed　that　the　release　process　of　PCBs　from　food　matrix　conformed　by　the　pseudo-second-order　kinetic　model　at　non-equilibrium　condition　and　reached　equilibrium　after　2h　in　small　intestine,　while　the　adsorption　and　release　processes　at　equilibrium　were　follow　Langmuir　isotherm　equation　(R-2＞0.99).　The　study　results　also　demonstrated　that　the　relationship　between　the　released　PCBs　and　their　levels　in　food　is　a　partitioning-based　model,　and　has　nothing　to　do　with　their　contamination　levels.　The　present　study　provides　basic　information　for　advanced　in　vitro　and　in　vivo　studies　on　the　mechanism　or　uptake　of　PCBs　or　other　chemicals　in　human　body.