In　the　present　study,　the　cytotoxic　effects　and　toxicological　mechanism　of　six　polybrominated　diphenyl　ethers　(PBDEs)　metabolites　(3-OH-BDE47,　3-MeO-BDE47,　5-OH-BDE47,　5-MeO-BDE47,　6-OH-BDE85　and　6-MeO-BDE85)　on　L02　cells　were　explored　by　investigating　the　cell　viability,　apoptosis,　lactic　dehydrogenase　(LDH)　leakage,　and　oxidative　stress　response.　The　results　showed　that　these　metabolites　could　inhibit　cell　proliferation　and　induce　apoptosis,　among　which　6-OH-BDE85　had　the　highest　efficiency.　LDH　leakage　test　also　showed　that　6-OH-BDE85　had　the　strongest　ability　to　cause　LDH　release.　The　reactive　oxygen　species　(ROS)　levels　in　6-OH-BDE85-　and　3-OH-BDE47-treated　groups　were　significantly　elevated　in　a　dose-dependent　manner.　After　treatment　for　24　h,　four　BDE47　metabolites　(3-OH-BDE47,　3-MeO-BDE47,　5-OH-BDE47,　and　5-MeO-BDE47)　induced　an　increase　in　superoxide　dismutase　(SOD)　activity　and　decrease　in　glutathione　(GSH)　level,　whereas　6-OH-BDE85　led　to　a　decrease　in　both　SOD　activity　and　GSH　level.　These　effects　disappeared　after　continued　culturing　for　another　24　h.　In　conclusion,　these　PBDE　metabolites,　especially　6-OH-BDE85,　showed　cytotoxicity　on　L02　cells,　which　was　at　least　partially　related　to　the　oxidative　stress　level.