Indexed by:
Abstract:
We have proposed a signal dual-amplification electrochemiluminescence (ECL) strategy based on tris(2,2 '- bipyridyl)ruthenium(II) (Ru(bpy)32+) as chromophores confined with three-dimensional (3D) zinc oxalate metal-organic frameworks (Ru(bpy)32+@MOFs) for the detection of miRNA-21. The three-dimensional chromophore connectivity in zinc oxalate MOFs provided a network among Ru(bpy)32+ units, shielding the chromophores from solvent molecules and resulting in high Ru(II) complex emission efficiency. Additionally, we discovered that magnetic beads (MBs) used as carrier for enriched signals contribute to enhanced ECL intensity of the chromophore. To evaluate its clinical application, we applied this method to determine the concentration of miRNA21 solutions ranging from 1.56 to 100 nM, obtaining a calibration curve of ECL intensity versus logarithm of concentration (logC) of miRNA-21 with a high correlation coefficient. This work demonstrates the construction of a signal amplification strategy ECL biosensor for miRNA using Ru(bpy)32+@MOF systems and its application in ECL detection for analyte methodology.
Keyword:
Reprint 's Address:
Email:
Version:
Source :
TALANTA
ISSN: 0039-9140
Year: 2023
Volume: 268
5 . 6
JCR@2023
5 . 6 0 0
JCR@2023
JCR Journal Grade:1
CAS Journal Grade:1
Cited Count:
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 2
Affiliated Colleges: