• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
成果搜索

author:

Zheng, Kai (Zheng, Kai.) [1] | Pan, Jiangfei (Pan, Jiangfei.) [2] | Yu, Zipei (Yu, Zipei.) [3] | Yi, Changqing (Yi, Changqing.) [4] | Li, Mei-Jin (Li, Mei-Jin.) [5] (Scholars:李梅金)

Indexed by:

EI Scopus SCIE

Abstract:

We have proposed a signal dual-amplification electrochemiluminescence (ECL) strategy based on tris(2,2 '- bipyridyl)ruthenium(II) (Ru(bpy)32+) as chromophores confined with three-dimensional (3D) zinc oxalate metal-organic frameworks (Ru(bpy)32+@MOFs) for the detection of miRNA-21. The three-dimensional chromophore connectivity in zinc oxalate MOFs provided a network among Ru(bpy)32+ units, shielding the chromophores from solvent molecules and resulting in high Ru(II) complex emission efficiency. Additionally, we discovered that magnetic beads (MBs) used as carrier for enriched signals contribute to enhanced ECL intensity of the chromophore. To evaluate its clinical application, we applied this method to determine the concentration of miRNA21 solutions ranging from 1.56 to 100 nM, obtaining a calibration curve of ECL intensity versus logarithm of concentration (logC) of miRNA-21 with a high correlation coefficient. This work demonstrates the construction of a signal amplification strategy ECL biosensor for miRNA using Ru(bpy)32+@MOF systems and its application in ECL detection for analyte methodology.

Keyword:

Electrochemiluminescence MiRNA Smartphone-assisted detection Zinc oxalate metal-organic frameworks

Community:

  • [ 1 ] [Zheng, Kai]Fuzhou Univ, Dept Chem, Key Lab Anal & Detect Technol Food Safety, Minist Educ & Fujian Prov, Fuzhou, Peoples R China
  • [ 2 ] [Li, Mei-Jin]Fuzhou Univ, Dept Chem, Key Lab Anal & Detect Technol Food Safety, Minist Educ & Fujian Prov, Fuzhou, Peoples R China
  • [ 3 ] [Pan, Jiangfei]Sun Yat Sen Univ, Sch Biomed Engn, Key Lab Sensing Technol & Biomed Instruments Guang, Guangzhou 510006, Peoples R China
  • [ 4 ] [Yu, Zipei]Sun Yat Sen Univ, Sch Biomed Engn, Key Lab Sensing Technol & Biomed Instruments Guang, Guangzhou 510006, Peoples R China
  • [ 5 ] [Yi, Changqing]Sun Yat Sen Univ, Sch Biomed Engn, Key Lab Sensing Technol & Biomed Instruments Guang, Guangzhou 510006, Peoples R China
  • [ 6 ] [Yi, Changqing]Sun Yat Sen Univ Shenzhen, Res Inst, Shenzhen 518057, Peoples R China

Reprint 's Address:

  • [Li, Mei-Jin]Fuzhou Univ, Dept Chem, Key Lab Anal & Detect Technol Food Safety, Minist Educ & Fujian Prov, Fuzhou, Peoples R China;;[Yi, Changqing]Sun Yat Sen Univ Shenzhen, Res Inst, Shenzhen 518057, Peoples R China;;

Show more details

Related Keywords:

Source :

TALANTA

ISSN: 0039-9140

Year: 2023

Volume: 268

5 . 6

JCR@2023

5 . 6 0 0

JCR@2023

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

Online/Total:109/10011692
Address:FZU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350116) Contact Us:0591-22865326
Copyright:FZU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备05005463号-1