Colorectal　cancer　is　a　prevalent　malignancy　globally,　often　linked　to　chronic　colitis.　Terahertz　technology,　with　its　noninvasive　and　fingerprint　spectroscopic　properties,　holds　promise　in　disease　diagnosis.　This　study　aimed　to　explore　terahertz　technology＇s　application　in　colitis-associated　cancer　using　a　mouse　model.　Mouse　colorectal　tissues　were　transformed　into　paraffin-embedded　blocks　for　histopathological　analysis　using　HE　staining.　Terahertz　transmission　spectroscopy　was　performed　on　the　tissue　blocks.　By　comparing　terahertz　absorption　differences,　specific　frequency　bands　were　identified　as　optimal　for　distinguishing　cancerous　and　normal　tissues.　The　study　revealed　that　terahertz　spectroscopy　effectively　differentiates　colitis-related　cancers　from　normal　tissues.　Remarkably,　1.8　THz　emerged　as　a　potential　optimal　frequency　for　diagnosing　colorectal　cancer　in　mice.　This　suggests　the　potential　for　rapid　histopathological　diagnosis　of　colorectal　cancer　using　terahertz　technology.　This　graphical　abstract　demonstrates　the　absorption　differences　between　mouse　colitis-associated　bowel　cancer　tissues　and　normal　tissues　in　the　terahertz　frequency　range.　Analysis　of　terahertz　transmission　spectroscopy　reveals　that　mouse　bowel　cancer　tissues　absorb　significantly　more　terahertz　waves　than　normal　tissues　in　the　range　of　0.2-2　THz,　exhibiting　peak　absorption　between　1.6　and　1.8　THz.　Further　zonal　analysis　showed　that　the　difference　in　absorption　coefficients　between　intestinal　cancer　tissues　and　normal　tissues　was　greatest　in　the　range　of　1.4-2.0　THz,　and　the　maximum　difference　was　reached　at　the　frequency　of　1.8　THz,　which　may　become　the　optimal　terahertz　frequency　for　the　diagnosis　of　intestinal　cancer　in　mice.　These　findings　reveal　the　potential　application　of　terahertz　spectral　analysis　in　the　detection　of　colitis-associated　intestinal　cancer.image