Herein,　magnetic　electrochemiluminescence　(ECL)　nanoparticle　Fe3O4@PtPd/Ru(bpy)32+　had　been　synthesized　then　been　coupled　with　CRISPR/Cas13a　system　and　Zn2+　dependent　DNAzyme　to　design　a　novel　ECL　biosensor　for　specific　detection　of　microRNA-145　(miRNA).　The　synthesized　multifunctional　magnetic　nanoluminescent　materials　Fe3O4@PtPd/Ru(bpy)32+　not　only　load　Ru(bpy)32+　to　provide　ECL　signals,　but　also　can　quickly　achieve　separation　and　enrichment　from　complex　matrices.　In　addition,　ferrocene　(Fc)　was　used　as　a　quencher　in　the　Ru(bpy)32+/tripropylamine　(TPA)　system.　Fc　was　modified　on　DNA　bound　to　Fe3O4@PtPd.　Benefited　from　the　highly　specific　recognition　ability　of　CRISPR/Cas13a,　the　target　miRNA　induces　CRISPR/Cas13a　trans-cleavage　to　trigger　the　Zn2+-dependent　DNAzyme　cyclic　cleavage　to　realize　the　dual　signal　amplification.　DNA　modified　by　Fc　was　split　by　target　miRNA-induced　cleaving,　and　then　magnetic　separation　was　performed　to　keep　Fc　away　from　the　surface　of　the　nanoparticles.　Thus,　the　enhanced　ECL　signal　was　obtained　to　detect　miRNA-145.　Under　optimized　conditions,　the　prepared　sensor　showed　a　wide　linear　range　(1　fM　to　1　nM)　and　a　low　limit　of　detection　(LOD)　down　to　0.41　fM.　Furthermore,　it　shows　excellent　selectivity　and　good　reproducibility.　The　proposed　ECL　platform　has　huge　potential　applications　in　the　development　of　various　sensitive　sensors　for　detecting　the　other　miRNA.　©　2023