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author:

Zhang, Ying (Zhang, Ying.) [1] | Xu, Guoyan (Xu, Guoyan.) [2] | Lian, Guili (Lian, Guili.) [3] | Luo, Fang (Luo, Fang.) [4] (Scholars:罗芳) | Xie, Qunfang (Xie, Qunfang.) [5] | Lin, Zhenyu (Lin, Zhenyu.) [6] (Scholars:林振宇) | Chen, Guonan (Chen, Guonan.) [7] (Scholars:陈国南)

Indexed by:

EI Scopus SCIE

Abstract:

A novel electrochemiluminescence (ECL) biosensor was developed for high sensitive and selective detection of miRNA-21 based on the efficient and specific toehold-mediated strand displacement (TMSD) amplification with Ru(phen)(3)(2+) loaded DNA nanoclews (NCs-Ru(phen)(3)(2+)) as signal tags. The stable DNA nanoclews, synthesized by a simple rolling circle amplification reaction, were employed to load with Ru(phen)(3)(2+) efficiently as ECL signal tags to amplify the signals. As for TMSD, the substrate strand (Sub) was initially hybridized with P1 and P2 to form DNA duplex structures with a toehold 1. miRNA-21 could hybridize with the toehold 1 and trigger the TMSD amplification with the help of assist strand, releasing lots of P1 stands from DNA duplex structures. The TMSD technique realized the converting and amplification of the single miRNA-21 input to lots of output DNA (namely P1) with good selectivity, simultaneously. Output P1 were designed to expand the stem-locked region of HP, which were immobilized on the Au electrodes firstly. Subsequently, the opened HP could hybridize with the Ru(phen)(3)(2+), capturing the ECL signal tags closed to the sensing surface. The ECL intensity of the system had a linear relationship with the logarithm of the miRNA-21 concentration in the range of 1.0 fM to 100 pM with a limit of detection of 0.65 fM. The strategy was further applied to detect miRNA-21 in complex samples, and the result was consistent with the qRT-PCR.

Keyword:

DNA nanoclews Electrochemiluminescence biosensor miRNA-21 Ru(phen)(3)(2+) Toehold-mediated strand displacement

Community:

  • [ 1 ] [Zhang, Ying]Fujian Med Univ, Cent Lab, Affiliated Hosp 1, Fuzhou 350005, Fujian, Peoples R China
  • [ 2 ] [Xu, Guoyan]Fujian Med Univ, Dept Cadres Ward, Affiliated Hosp 1, Fuzhou 350005, Fujian, Peoples R China
  • [ 3 ] [Lian, Guili]Fujian Med Univ, Dept Cadres Ward, Affiliated Hosp 1, Fuzhou 350005, Fujian, Peoples R China
  • [ 4 ] [Xie, Qunfang]Fujian Med Univ, Dept Cadres Ward, Affiliated Hosp 1, Fuzhou 350005, Fujian, Peoples R China
  • [ 5 ] [Lin, Zhenyu]Fuzhou Univ, Coll Chem, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ,Key Lab Analyt Sci Food Safety & Biol, Fuzhou 350116, Fujian, Peoples R China
  • [ 6 ] [Chen, Guonan]Fuzhou Univ, Coll Chem, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ,Key Lab Analyt Sci Food Safety & Biol, Fuzhou 350116, Fujian, Peoples R China
  • [ 7 ] [Luo, Fang]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350116, Fujian, Peoples R China

Reprint 's Address:

  • 林振宇

    [Lin, Zhenyu]2 Xue Yuan Rd, Fuzhou, Fujian, Peoples R China;;[Xie, Qunfang]20 Chazhong Rd, Fuzhou, Fujian, Peoples R China

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Source :

BIOSENSORS & BIOELECTRONICS

ISSN: 0956-5663

Year: 2020

Volume: 147

1 0 . 6 1 8

JCR@2020

1 0 . 7 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:160

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 71

SCOPUS Cited Count: 71

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 4

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