Seed　germination　is　a　vital　process　in　plant　development　involving　dynamic　biochemical　transformations　such　as　lipid　metabolism.　However,　the　spatial　distribution　and　dynamic　changes　of　lipids　in　different　seed　compartments　during　germination　are　poorly　understood.　In　this　study,　we　employed　liquid　chromatography/mass　spectrometry　(LC/MS)-based　lipidomics　and　MALDI　mass　spectrometry　imaging　(MSI)　to　investigate　lipid　changes　occurring　in　the　cotyledon　and　plumule　of　mung　bean　seeds　during　germination.　Lipidomic　data　revealed　that　the　germination　process　reduced　the　levels　of　many　glycerolipids　(e.g.,　triglyceride)　and　phosphatidylglycerols　(e.g.,　phosphatidylcholine)　while　increased　the　levels　of　lysophospholipids　(e.g.,　lysophosphatidylcholine)　in　both　the　cotyledon　and　plumule.　Sphingolipids　(e.g.,　sphingomyelin)　displayed　altered　levels　solely　in　the　plumule.　Sterol　levels　increased　in　the　cotyledon　but　decreased　in　the　plumule.　Further　imaging　results　revealed　that　MALDI-MSI　could　serve　as　a　supplement　and　validate　LC-MS　data.　These　findings　enhance　our　understanding　of　the　metabolic　processes　underlying　seedling　development,　with　potential　implications　for　crop　improvement　and　seed　quality　control.　©　2023　The　Authors.　Published　by　American　Chemical　Society