Given　continuous　developments　in　industrial　and　scientific　research,　the　separation　and　analysis　of　complex　systems　with　high　sensitivity,　throughput,　and　selectivity　is　facing　new　challenges.　Chromatography　plays　an　irreplaceable　role　in　separation　science　and　is　widely　ap.　plied　in　environmental　monitoring,　pharmaceutical　analysis,　and　food　safety.　Owing　to　their　outstanding　advantages,　such　as　high　loading　capacity,　precise　quantification,　and　good　repro.　ducibility,　chromatographic　separation　techniques　based　on　various　retention　mechanisms　have　been　utilized　to　detect　different　analytes.　The　stationary　phase　is　the　core　material　of　chromato.　graphic　columns　and　has　an　extremely　important　influence　on　their　separation　performance.　The　selectivity　and　efficiency　of　separation　largely　depend　on　the　chromatographic　stationary　phase.　However,　traditional　stationary　phases,　such　as　silicon.based　matrices,　are　characterized　by　complex　preparation　processes,　poor　permeability,　large　mass　transfer　resistance,　and　a　nar.　row　pH　range.　In　addition,　polymer　matrices　show　poor　mechanical　stability　and　susceptibility　to　swelling,　which　limit　their　applications　in　the　field　of　separation.　Therefore,　the　development　of　novel　stationary　phases　with　the　advantages　of　traditional　stationary　phases　has　become　a　re.　search　emphasis　in　the　field　of　analytical　science　in　efforts　to　meet　separation　requirements　un.　der　different　environments.　Various　stationary　phases　based　on　novel　porous　materials,　such　as　metal　organic　frameworks　(　MOFs),　porous　organic　cages　(　POCs),　and　covalent　organic　frameworks　(　COFs),　are　used　for　chromatographic　separation.　As　mesh　crystalline　porous　ma.　terials,　MOFs　have　the　advantages　of　a　large　surface　area,　adjustable　structure,　and　easy　func.　tionalization;　thus,　they　are　widely　used　as　chromatographic　stationary　phases　in　reverse.　phase　chromatography,　hydrophilic.mode　chromatography,　mixed.　mode　chromatography,　and　other　separation　modes.　However,　because　the　pore　size　of　MOFs　is　small　and　most　MOFs　demon.　strate　poor　chemical　stability　under　acidic　or　alkaline　conditions,　their　applications　in　chroma.　tographic　separation　are　greatly　limited.　COFs　are　porous,　crystalline　polymer　materials　com.　posed　of　light　elements　(　H,　O,　C,　N,　B,　and　Si)　connected　via　covalent　bonds.　Their　advanta.　ges　include　a　low　density,　large　specific　surface　area,　high　porosity,　good　chemical　and　ther.　mal　stability,　regular　pores,　and　adjustable　pore　sizes.　Because　of　their　unique　structures　and　properties,　COFs　are　widely　used　in　many　fields　such　as　catalysis,　enrichment,　gas　capture,　and　sensing.　COF　materials　are　also　suitable　for　separation　analysis　and　considered　ideal　materi.　als　for　novel　chromatographic　stationary　phases.　This　review　summarizes　the　latest　research　progress　on　the　preparation　and　applications　of　COF.　based　chromatographic　stationary　phases　over　the　past　five　years.　First,　the　preparation　of　COF.　based　stationary　phases　(　SiO2　@　COFs　stationary　phase,　COFs　monolithic　stationary　phase,　pure　COFs　stationary　phase　and　COFs.　coated　stationary　phase)　is　introduced.　The　latest　applications　of　COF.　based　stationary　phases　in　the　separation　of　organic　compounds,　isomers,　and　chiral　compounds　are　then　described　in　detail.　Finally,　the　future　development　trends　and　challenges　of　chromatographic　stationary　phases　based　on　COFs　are　discussed　to　provide　new　ideas　for　the　future　design　and　develop.　ment　of　novel　chromatographic　stationary　phases　based　on　COFs.