The　disposal　of　discarded　vulcanized　natural　rubber　waste　has　presented　substantial　environmental　challenges.　This　study　aimed　to　investigate　the　degradation　of　vulcanized　natural　rubber　through　the　utilization　of　enriched　bacterial　consortia　with　NRE3　derived　from　the　soil.　The　highest　weight　loss　of　38.84　%　was　achieved　with　NRE3　after　four　weeks　of　incubation.　Additionally,　the　crosslinking　density　of　rubber　decreased　by　32.3　%　and　the　tensile　strength　decreased　from　69.1　to　9.5　MPa.　SEM　analysis　revealed　the　presence　of　significant　cracks　and　holes　on　the　surface　of　vulcanized　natural　rubber.　Moreover,　FT-IR　and　XPS　analyses　demonstrated　a　decrease　in　the　sulfur　(S)　and　carbon　(C)　content　of　the　rubber,　while　the　oxygen　(O)　content　increased.　The　concentration　of　SO42-　in　the　medium　increased　from　478.37　to　840.26　mg/L,　indicating　the　collapse　of　the　rubber　structure.　The　NMR　and　GC-MS　findings　indicated　the　presence　of　degradation　products,　specifically　polyisoprene　oligomers　and　terpenoids.　Through　high-throughput　sequencing　analyses,　Acinetobacter,　Chryseobacterium,　Stenotrophomonas　and　other　bacterial　were　discovered　in　NRE3.　Based　on　the　chemical　structure　characterizations　and　molecular　analyses,　a　degradation　pathway　was　proposed.　These　findings　pave　a　way　for　utilizing　rubberdegrading　microorganisms　present　in　soil　and　contribute　to　the　establishment　of　a　biodegradation　method　for　disposing　of　vulcanized　rubber　waste.