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author:

Zhang, Jianhui (Zhang, Jianhui.) [1] | Tan, Meirong (Tan, Meirong.) [2] | Chen, Qian (Chen, Qian.) [3] | Zhang, Kangyao (Zhang, Kangyao.) [4] | Zhou, Qian (Zhou, Qian.) [5] | Lai, Wenqiang (Lai, Wenqiang.) [6] | Tang, Dianping (Tang, Dianping.) [7] (Scholars:唐点平)

Indexed by:

EI Scopus SCIE

Abstract:

Carcinoembryonic antigen (CEA), a vital biomarker, plays a significant role in the early diagnosis and prognostic estimation of malignant tumors. In this study, a split-type photoelectrochemical immunoassay for the sensitive quantification of CEA has been successfully developed based on the target-induced in situ formation of a Z-type heterojunction. First, gold nanoparticle-decorated ZnIn2S4 (AuNPs/ZnIn2S4) composites were synthesized and used for the fabrication of photoelectrodes. Then, the detection antibody labeled with Ag nanoparticles was formed and applied for the biorecognition of CEA and subsequent liberation of Ag+ ions to induce the in situ formation of Ag2S/AuNPs/ZnIn2S4, a Z-type heterojunction, on the photoelectrode. The Z-type Ag2S/AuNPs/ZnIn2S4 heterojunction with effectively promoted separation of photogenerated charge carriers could lead to a markedly enhanced photocurrent response and highly sensitive quantification of CEA. Moreover, the three-dimensional spatial structure of ZnIn2S4 provides abundant active sites for the reaction and exhibits non-enzymatic properties, which are conducive to the further improvement of the analytical performance of CEA. The developed split-type photoelectrochemical immunoassay with good sensitivity, satisfactory selectivity, reliable stability, wide dynamic linear range (0.01-20 ng mL(-1)), and low detection limit (7.3 pg mL(-1)) offers valuable insights into the development of novel PEC biosensing models for the detection of tumor biomarkers and holds potential application value in the field of disease diagnosis.

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Community:

  • [ 1 ] [Zhang, Jianhui]Fuzhou Univ, Sch Adv Mfg, Jinjiang 362200, Peoples R China
  • [ 2 ] [Tan, Meirong]Fuzhou Univ, Sch Adv Mfg, Jinjiang 362200, Peoples R China
  • [ 3 ] [Chen, Qian]Fuzhou Univ, Sch Adv Mfg, Jinjiang 362200, Peoples R China
  • [ 4 ] [Zhang, Kangyao]Fuzhou Univ, Sch Adv Mfg, Jinjiang 362200, Peoples R China
  • [ 5 ] [Zhou, Qian]Henan Univ, Coll Chem & Mol Sci, Henan Int Joint Lab Med Plants Utilizat, Kaifeng 475000, Peoples R China
  • [ 6 ] [Lai, Wenqiang]Minnan Normal Univ, Coll Chem Chem Engn & Environm, Key Lab Modern Analyt Sci & Separat Technol Fujian, Key Lab Pollut Monitoring & Control Fujian Prov, Zhangzhou 363000, Peoples R China
  • [ 7 ] [Tang, Dianping]Fuzhou Univ, Inst Nanomed & Nanobiosensing, Key Lab Anal & Detect Food Safety, Dept Chem,Minist Educ & Fujian Prov, Fuzhou 350108, Peoples R China

Reprint 's Address:

  • 张康耀

    [Zhang, Kangyao]Fuzhou Univ, Sch Adv Mfg, Jinjiang 362200, Peoples R China;;[Zhou, Qian]Henan Univ, Coll Chem & Mol Sci, Henan Int Joint Lab Med Plants Utilizat, Kaifeng 475000, Peoples R China

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Source :

ANALYTICAL METHODS

ISSN: 1759-9660

Year: 2024

Issue: 13

Volume: 16

Page: 1901-1907

2 . 7 0 0

JCR@2023

Cited Count:

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ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

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30 Days PV: 0

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