Enzyme　mimics　with　dual　enzyme-like　activities　can　catalyse　cascade　reactions　with　high　efficiency　and　thus　play　a　significant　role　in　biochemistry　since　multistep　cascade　reactions　often　occur　in　biocatalysis.　Especially,　a　nanozyme　that　simultaneously　possesses　peroxidase-like　and　pyranose　oxidase　(POx)-like　activities　is　highly　desired　since　it　can　be　used　for　bio-synthetizing　rare　sugars　and　fabricating　bio-enzyme-free　colorimetric　methods　for　the　detection　of　various　pyranoses.　We　herein　prepared　a　novel　dual-active　nanozyme,　which　simultaneously　possesses　enhanced　and　stable　peroxidase-like　and　POx-like　activities,　by　loading　gold　nanoparticles　(AuNPs)　on　MoS2　nanosheets　(AuNPs@MoS2).　The　prepared　AuNPs@MoS2　nanozyme　can　catalyse　various　tandem　reactions　of　pyranose　oxidation　and　H2O2-mediated　oxidation　of　TMB　with　high　efficiency　and,　therefore,　can　be　used　to　fabricate　bio-enzyme-free　colorimetric　methods　for　the　detection　of　various　monosaccharides　with　a　pyranyl　ring,　including　glucose,　xylose　and　galactose.　Based　on　the　AuNPs@MoS2　nanozyme,　we　successfully　developed　bio-enzyme-free　colorimetric　methods　for　the　detection　of　glucose,　xylose　and　galactose　with　a　visual　detection　limit　of　0.2-0.3　mM　and　a　spectrometry　detection　limit　of　5.0-11　mu　M.　The　developed　colorimetric　glucose,　xylose　and　galactose　detection　methods　were　successfully　used　to　detect　glucose　in　serum,　xylose　in　bread　and　galactose　in　milk,　respectively,　with　a　recovery　of　89-108%　and　a　relative　standard　deviation　(RSD,　n　=　5)　of　＜5%.　With　enhanced　peroxidase-like　and　POx-like　activities　and　good　stability,　the　developed　AuNPs@MoS2　provided　a　promising　dual-active　nanozyme　for　the　bio-enzyme-free　catalysis　of　various　cascade　reactions　for　the　oxidation　of　various　monosaccharides　with　a　pyranyl　ring　and　for　further　fabricating　bio-enzyme-free,　cost-effective　and　simple　colorimetric　sensors　for　the　visual　detection　of　various　monosaccharides　with　a　pyranyl　ring,　including　glucose,　xylose　and　galactose.