The　polymorphic　formation　of　G-quadruplex　brings　more　attentions,　because　changes　of　formation　sites　affect　many　physiological　processes,　like　telomere　maintenance,　transcription　and　DNA　replication.　These　processes　are　highly　susceptible　to　ROS-induced　oxidative　damage.　In　this　work,　bichromophoric　cores　of　symmetric　squaraine　probe　SQ　linked　with　ether　chain　is　conducive　to　the　recognition　of　G-quadruplex,　based　on　the　mechanism　of　intra　or　intermolecular　“aggregation-disaggregation”.　Several　interaction　behaviors,　such　as　self-aggregation,　binding　affinity,　stoichiometric　ratio,　were　fully　investigated　via　spectrum　titration,　circular　dichroism,　fluorescent　intercalator　displacement　assay,　and　molecular　docking.　Furthermore,　tracking　G-quadruplex　distribution　in　the　cytoplasm　was　monitored　by　SQ　during　the　oxidative　stress　enhancement.　Interestingly,　the　G-quadruplex　levels　in　the　cytoplasm　of　cancer　cells　were　slightly　higher　than　that　of　normal　cells　to　deal　with　oxidation-reduction　imbalance.　Overall,　the　lower　detection　limit　of　SQ　for　parallel　G-quadruplex　like　pu27　is　as　low　as　7.2　nM;　and　the　complex　of　SQ　and　G-quadruplex　will　not　change　the　topological　structure　of　G-quadruplex.　It　has　good　sensitive　and　stability　for　parallel　type　of　G-quadruplex.　It　is　a　potential　tool　for　bioimaging　of　G-quadruplex　in　the　cytoplasm.　©　2024　Elsevier　Ltd