The　polymorphic　formation　of　G-quadruplex　brings　more　attentions,　because　changes　of　formation　sites　affect　many　physiological　processes,　like　telomere　maintenance,　transcription　and　DNA　replication.　These　processes　are　highly　susceptible　to　ROS-induced　oxidative　damage.　In　this　work,　bichromophoric　cores　of　symmetric　squaraine　probe　SQ　linked　with　ether　chain　is　conducive　to　the　recognition　of　G-quadruplex,　based　on　the　mechanism　of　intra　or　intermolecular　＂aggregation-disaggregation＂.　Several　interaction　behaviors,　such　as　self-aggregation,　binding　affinity,　stoichiometric　ratio,　were　fully　investigated　via　spectrum　titration,　circular　dichroism,　fluorescent　intercalator　displacement　assay,　and　molecular　docking.　Furthermore,　tracking　G-quadruplex　distribution　in　the　cytoplasm　was　monitored　by　SQ　during　the　oxidative　stress　enhancement.　Interestingly,　the　Gquadruplex　levels　in　the　cytoplasm　of　cancer　cells　were　slightly　higher　than　that　of　normal　cells　to　deal　with　oxidation-reduction　imbalance.　Overall,　the　lower　detection　limit　of　SQ　for　parallel　G-quadruplex　like　pu27　is　as　low　as　7.2　nM;　and　the　complex　of　SQ　and　G-quadruplex　will　not　change　the　topological　structure　of　G-quadruplex.　It　has　good　sensitive　and　stability　for　parallel　type　of　G-quadruplex.　It　is　a　potential　tool　for　bioimaging　of　G-quadruplex　in　the　cytoplasm.