T-2　toxin,　a　hazardous　mycotoxin　often　present　in　cereals　and　products　based　on　cereals,　poses　a　substantial　risk　to　humans　and　animals　due　to　its　high　toxicity.　The　development　of　uncomplicated,　quick　and　highly　sensitive　methods　for　detecting　T-2　toxin　is　imperative.　In　this　work,　a　portable　sensing　system　was　constructed　using　water　column　height　as　a　readout　device　in　combination　with　a　controlled　release　system,　which　allows　for　an　accurate　quantitative　analysis　of　T-2　toxin　without　the　need　for　expensive　instrumentation　or　skilled　technicians.　Hyaluronic　acid　(HA)　hydrogel　was　constructed　by　double　cross-linked　DNA/aptamer　hybrids　with　polyethyleneimine　(PEI)　and　embedded　with　platinum　nanoparticles　(Pt　NPs).　The　aptamer　specifically　bound　to　T-2　toxin　in　its　presence,　resulting　in　the　disruption　of　the　hydrogel　and　subsequent　release　of　the　Pt　NPs.　These　Pt　NPs　were　later　mixed　with　a　solution　of　H2O2　in　a　confined　reaction　flask,　leading　to　the　decomposition　of　H2O2　into　O2.　A　glass　capillary　tube　containing　a　column　of　red　water　had　been　inserted　into　the　cap　of　the　reaction　flask,　and　the　low　solubility　of　O2　led　to　an　increase　in　pressure　within　the　reaction　unit,　causing　the　red　water　column　to　rise.　There　is　a　good　linear　correlation　between　the　height　of　the　capillary　liquid　level　and　the　T-2　toxin　concentration　in　the　range　of　20　ng/mL　to　6　μg/mL.　The　system　has　been　successfully　used　to　detect　T-2　toxin　in　samples　of　barley　tea　and　corn.　©　2024　Elsevier　B.V.