With　the　increasing　globalization　of　the　food　supply,　foodborne　infection　and　food　poisoning　brought　by　Staphylococcus　aureus　(S.　aureus)　have　progressively　become　intractable　issues.　This　work　aimed　to　research　the　dual　inhibitory　effects　of　zinc-binding　peptides　(termed　CSSP-Zn)　on　both　planktonic　and　biofilm　of　S.　aureus　6538,　with　respect　to　its　utilization　on　pork　models.　Further　research　showed　CSSP-Zn　could　disrupt　the　cytomembrane　of　permeability　and　integrity,　triggering　cytoplasm　overflow,　thereby　causing　proton　motive　force　(PMF)　collapse　and　metabolic　disorder　in　planktonic　strain　6538.　The　antibiofilm　mechanism　of　CSSP-Zn　primarily　involved　disrupting　the　expression　of　polysaccharide　intercellular　adhesion　(PIA)　biosynthesis-related　genes　(e.g.　icaA,　icaB,　icaC　and　icaD),　causing　an　enervated　intercellular　adhesion　and　lessened　biofilm　viability.　When　employed　to　a　pork　model,　CSSP-Zn　could　obviously　attenuate　strain　6538　colonization　and　enterotoxin　secretion.　These　results　not　only　demonstrated　the　practical　antibacterial　efficacy　of　CSSP-Zn　but　also　offered　a　novel　strategy　for　mitigating　the　risk　of　S.　aureus　contamination　in　pork.　©　2024　Elsevier　Ltd