With　the　increasing　globalization　of　the　food　supply,　foodborne　infection　and　food　poisoning　brought　by　Staphylococcus　aureus　(　S.　aureus　)　have　progressively　become　intractable　issues.　This　work　aimed　to　research　the　dual　inhibitory　effects　of　zinc　-binding　peptides　(termed　CSSP-Zn)　on　both　planktonic　and　biofilm　of　S.　aureus　6538,　with　respect　to　its　utilization　on　pork　models.　Further　research　showed　CSSP-Zn　could　disrupt　the　cytomembrane　of　permeability　and　integrity,　triggering　cytoplasm　overflow,　thereby　causing　proton　motive　force　(PMF)　collapse　and　metabolic　disorder　in　planktonic　strain　6538.　The　antibiofilm　mechanism　of　CSSP-Zn　primarily　involved　disrupting　the　expression　of　polysaccharide　intercellular　adhesion　(PIA)　biosynthesis　-related　genes　(e.g.　icaA　,　icaB　,　icaC　and　icaD　),　causing　an　enervated　intercellular　adhesion　and　lessened　biofilm　viability.　When　employed　to　a　pork　model,　CSSP-Zn　could　obviously　attenuate　strain　6538　colonization　and　enterotoxin　secretion.　These　results　not　only　demonstrated　the　practical　antibacterial　efficacy　of　CSSP-Zn　but　also　offered　a　novel　strategy　for　mitigating　the　risk　of　S.　aureus　contamination　in　pork.