A　phenyl-modified　silica　isoporous　membrane　(Ph-SIM)　was　prepared　on　the　indium-tin-oxide　(ITO)　electrode　using　the　electrochemically　assisted　self-assembly　(EASA)　method.　The　resulting　Ph-SIM　preserved　vertically　ordered　nanochannels　while　exhibiting　outstanding　hydrophobicity　due　to　the　incorporation　of　phenyl　groups　within　the　nanochannels.　As　a　result,　the　Ph-SIM/ITO　sensor　exhibited　a　remarkable　affinity　for　PCNB　extraction　through　hydrophobic　interactions,　leading　to　high　detection　sensitivity.　The　electrochemical　response　showed　a　linear　enhancement　with　the　logarithmic　concentration　of　PCNB　ranging　from　0.1　to　20.0 µM,　and　the　limit　of　detection　was　4.64 nM.　Practical　results　demonstrated　that　the　Ph-SIM/ITO　sensor　possessed　good　anti-fouling　capability　and　robust　stability,　making　it　a　promising　candidate　for　portable　detection　of　non-polar　contaminants.　Graphical　Abstract:　(Figure　presented.)　©　The　Author(s),　under　exclusive　licence　to　Springer-Verlag　GmbH　Austria,　part　of　Springer　Nature　2024.