Bacterial　infections　result　in　significant　burdens　on　public　health,　especially　with　the　increasing　prevalence　of　antibiotic　resistance　owing　to　the　overuse　of　antibiotics.　The　development　of　the　next-generation　nanoantibacterial　materials　as　alternatives　to　antibiotics　is　urgently　needed.　Metal-organic　frameworks　(MOFs)　have　been　emerging　as　promising　antibacterial　materials.　However,　the　impact　of　metal　centers　on　the　properties　and　antibacterial　activity　of　MOFs　has　not　been　clarified　to　date.　In　this　work,　five　M-MOF　nanomaterials　(M　=　Fe,　Co,　Ni,　Cu,　Zn)　are　synthesized　with　2-methylimidazole　as　an　organic　ligand.　Subsequently,　the　minimum　inhibitory　concentration　(MIC),　minimum　bactericidal　concentration,　and　time-kill　curves　are　studied　to　evaluate　their　antibacterial　activity.　In　addition,　the　destruction　of　the　bacterial　cells　after　treatment　with　M-MOFs　is　observed　via　scanning　electron　microscopy.　The　experimental　results　demonstrate　that　the　Co-MOF　and　Zn-MOF　polyhedra　exhibit　optimal　antibacterial　activity.　They　can　effectively　inhibit　the　growth　of　both　Gram-negative　bacteria　Escherichia　coli　and　Gram-positive　bacteria　Staphylococcus　aureus　at　a　low　concentration.　On　the　other　hand,　the　Fe-MOF　irregular　particles　show　the　weakest　antibacterial　activity　among　five　M-MOFs　and　the　antibacterial　activity　of　the　Ni-MOF　and　Cu-MOF　nanosheets　are　comparable　to　each　other.　The　huge　difference　in　antibacterial　activity　of　M-MOFs　is　attributed　to　the　difference　in　the　shape　and　size,　specific　surface　area,　surface　charge,　ion　release,　and　production　of　reactive　oxygen　species.　Overall,　this　study　clarifies　the　relationship　between　metal　centers　in　M-MOFs　and　their　antibacterial　activity.