Green　tea　residue　(GTR)　contains　a　high　protein　content.　However,　the　protein　in　GTR　can＇t　be　effectively　extracted　using　traditional　methods.　Thus,　a　novel　method　using　ethylenediamine　tetraacetic　acid　(EDTA),　ammonium　oxalate,　or　Celluclast®　1.5　L　were　used　to　disperse　leaf　tissues　and　to　collect　mesophyll　cells　to　enrich　the　protein.　Compared　with　EDTA　or　ammonium　oxalate　treatment,　Celluclast®　1.5　L　treatment　achieved　the　highest　amounts　of　mesophyll　cells,　about　2.7　×　106　g−1　of　GTR.　The　number　of　collected　mesophyll　cells　was　positively　and　linearly　correlated　with　the　extraction　rate　of　glucose　and　xylose,　indicating　that　cellulose　and　hemicellulose　were　key　components　influencing　cell　collection.　Celluclast®　1.5　L　treatment　enriched　the　protein　content　by　1.65　times　in　collected　mesophyll　cells　to　50%　protein　content　with　a　protein　recovery　of　88%,　providing　a　novel　scheme　to　obtain　high-quality　leaf　protein　for　the　food　industry.　©　2024