Selenium-enriched　probiotics　have　attracted　much　attention　due　to　the　physiological　activities　of　both　probiotics　and　selenium　(organic　selenium).　In　this　study,　we　investigated　the　mitigating　effect　of　selenium-enriched　Lactobacillus　rhamnosus　GG　(LGG@Se)　and　its　pathway　on　alcohol-induced　liver　injury　(ALI)　in　mice.　The　results　showed　that　LGG@Se　was　superior　to　LGG　and　sodium　selenite　in　alleviating　ALI.　Oral　LGG@Se　effectively　prevented　lipid　metabolism　disorders　and　liver　oxidative　damage　in　mice　caused　by　excessive　alcohol　intake.　16S　amplicon　sequencing　showed　that　LGG@Se　intervention　increased　the　abundance　of　beneficial　bacteria　and　suppressed　the　growth　of　harmful　bacteria　in　the　intestinal　tract　of　over-drinking　mice,　and　thus　effectively　modulated　the　homeostasis　of　intestinal　flora,　which　were　highly　correlated　with　the　improvement　of　liver　function.　Liver　metabolomics　analysis　indicated　that　LGG@Se　intervention　altered　liver　metabolic　profiling,　and　the　characteristic　biomarkers　were　mainly　involved　in　amino　acid　metabolism,　including　alanine,　aspartate　and　glutamate　metabolism,　arginine　biosynthesis,　etc.　In　addition,　LGG@Se　intervention　modulated　the　expression　of　genes　and　proteins　related　to　lipid　metabolism　and　oxidative　stress　in　liver　of　over-drinking　mice.　Western　blot　analysis　revealed　that　LGG@Se　intervention　up-regulated　the　expression　of　intestinal　barrier　function-related　proteins,　thereby　ameliorating　alcohol-induced　intestinal　barrier　damage.　Collectively,　these　findings　provide　scientific　evidence　that　LGG@Se　possesses　the　biological　activity　of　improving　alcohol-induced　lipid　metabolism　and　intestinal　microbiota　disorder.