The　behavior　of　high-strength　bolted　connection　(HSBC)　with　over-preload　tightening　torque　under　double　shear　test　was　presented　in　the　investigation.　A　total　of　108　HSBC　specimens　were　used　for　double　shear　test.　The　two　kinds　of　high-strength　bolt　grades　of　8.8　s　and　10.9　s　were　set　with　three　kinds　of　bolt　shank　diameters　of　16,　18　and　20　mm　which　were　used　in　this　study.　There　were　96　HSBC　specimens　with　different　over-preload　tightening　torque　and　12　HSBC　specimens　with　standard　torque　as　the　control　group.　The　over-preload　tightening　torque,　high-strength　bolt　grade,　bolt　shank　diameter,　and　steel　plate　surface　treatment　were　set　as　the　main　variables　for　the　double　shear　test　in　the　investigation.　This　study　analyzed　the　influences　of　main　variables　on　the　failure　mode,　strain　distribution,　anti-sliding　bearing　capacity,　ultimate　capacity,　shear　stiffness,　and　ductility　of　HSBC.　The　test　results　indicated　that　anti-sliding　bearing　capacity　of　HSBC　was　increased　greatly　with　the　increase　of　preload　tightening　torque.　However,　effects　of　over-preload　tightening　torque　on　the　ultimate　capacity　were　not　obvious.　In　addition,　this　investigation　provided　the　calculated　formula　to　calculate　the　ultimate　capacity　of　HSBC　with　over-preload　tightening　torque　under　the　double　test.　It　could　be　found　that　the　calculated　value　of　the　ultimate　capacity　was　in　good　agreement　with　the　test　value.　Furthermore,　the　finite　element　model　was　provided　to　simulate　and　analyze　the　shear　resistance　behavior　of　HSBC　with　over-preload　tightening　torque.