The　escalating　presence　of　antibiotic　contaminants　in　wastewater　presents　substantial　environmental　and　public　health　challenges,　primarily　due　to　their　role　in　the　proliferation　of　antibiotic　resistance　genes　(ARGs).　This　study　examines　the　effectiveness　of　a　hybrid　system　integrating　nano　zerovalent　iron　(nZVI)　and　layered　double　hydroxides　(LDH)　in　treating　wastewater　contaminated　with　ciprofloxacin　(CIP).　Reactor　experiments　revealed　that　incorporating　nZVI@LDH　mitigated　the　shock　caused　by　CIP　while　sustaining　a　methane　production　rate　that　was　116　%　higher　than　that　of　the　control　group.　Furthermore,　there　was　a　50　%　increase　in　CIP　removal　efficiency.　Notably,　there　was　a　significant　enrichment　of　hydrogenotrophic　methanogens,　such　as　Methanobacterium　and　Methanolinea,　in　the　nZVI@LDH-enhanced　reactors.　Additionally,　the　levels　of　reactive　oxygen　species　decreased　by　50　%,　from　11,813　±　1230　to　4525　±　1030　counts/s,　and　the　abundance　of　ARGs　declined　by　75–88　%　compared　to　the　control　reactors.　An　external　electric　field　further　promoted　electron　transfer,　boosting　the　relative　abundance　of　electrochemically　active　bacteria,　with　Proteobacteria　comprising　up　to　40　%　of　the　microbial　community　in　the　1　V　+　nZVI@LDH　reactor.　This　hybrid　system　demonstrates　significant　efficacy　in　degrading　CIP　and　decreasing　ARGs　generation,　underscoring　its　potential　as　a　sustainable　strategy　for　managing　antibiotic-laden　wastewater.　©　2025　Elsevier　B.V.