Anti-fouling　behavior　of　polyvinylidene　fluoride　(PVDF)　membrane　in　membrane　bioreactors　(MBRs)　can　be　enhanced　by　improving　its　physicochemical　properties.　To　evaluate　the　membrane　anti-fouling　ability,　this　study　comprehensively　investigated　the　effect　of　variation　in　fabrication　parameters　(i.e.　PVDF　and　polyvinylpyrrolidone　(PVP)　concentration,　scraper　clearance　and　evaporation　time)　on　membrane　properties,　especially　critical　flux.　The　result　illustrated　that　membrane　properties　could　be　significantly　improved　(p　＜　0.05)　with　a　lower　PVDF　concentration,　appropriate　PVP　concentration　and　prolonged　evaporation　time.　Under　the　optimal　parameter　(9　wt%　PVDF,　5　wt%　PVP,　40　s　evaporation　time　and　200　mu　m　scraper　clearance),　the　membrane　was　obtained　with　porous　structure　(68.16　f　0.49　%　of　porosity),　guaranteed　permeability　(3316.85　f　32.74　L/(m(2)&　sdot;h)　of　pure　water　flux)　and　improved　hydrophilicity　(59.33　f　0.39　degrees　of　contact　angle).　The　membrane　with　high　porosity　and　pure　water　flux　was　more　likely　to　enhance　its　critical　flux　through　correlation　analysis,　and　the　optimized　membrane　also　achieved　the　highest　critical　flux　(42.35　f　4.65　L/(m(2)&　sdot;h)).　Its　anti-fouling　performance　was　verified　successfully　in　anaerobic-anoxic-oxic　MBR　system　compared　with　the　selected　commercial　membrane　for　long-term　operation　(41　days)　and　lower　membrane　resistance　(214.52　x　10(7)　m(-1)).