As　a　probiotic　strain　isolated　from　Hongqu　rice　wine　(a　traditional　Chinese　fermented　food),　Limosilactobacillus　fermentum　FZU501　(designated　as　Lf)　demonstrates　exceptional　gastric　acid　and　bile　salt　tolerance,　showing　potential　application　as　a　functional　food.　The　aim　of　this　study　was　to　investigate　the　protective　effect　of　dietary　Lf　intervention　on　alcohol-induced　liver　injury　(ALI)　in　mice.　The　results　demonstrated　that　oral　administration　of　Lf　effectively　ameliorated　alcohol-induced　lipid　metabolism　disorders　by　reducing　the　serum　levels　of　TC,　TG　and　LDL-C　and　increasing　the　serum　levels　of　HDL-C.　In　addition,　oral　administration　of　Lf　effectively　prevented　alcohol-induced　liver　damage　by　increasing　the　hepatic　activities　of　antioxidant　enzymes　(CAT,　SOD,　GSH-Px)　and　alcohol-metabolizing　enzymes　(ADH　and　ALDH).　Interestingly,　16S　amplicon　sequencing　showed　that　oral　administration　of　Lf　increased　the　number　of　Prevotella,　Lachnospiraceae_NK4A136_group　and　Lactobacillus,　but　decreased　the　proportion　of　Faecalibaculum,　Adlercreutzia　and　Alistipes　in　the　intestines　of　mice　that　consumed　excessive　alcohol,　which　was　highly　associated　with　improved　liver　function.　As　revealed　by　liver　untargeted　metabolomics　studies,　oral　Lf　clearly　changed　liver　metabolic　profiles,　with　the　signature　biomarkers　mainly　involving　purine　metabolism,　taurine　metabolism,　tryptophan,　alanine,　aspartic　acid　and　glutamate　metabolism,　etc.　Additionally,　Lf　intervention　regulated　liver　gene　transcription　in　over-drinking　mice　for　cholesterol　metabolism,　bile　acid　metabolism,　fatty　acid　beta-oxidation,　alcohol　metabolism　and　oxidative　stress.　Taken　together,　the　above　research　results　provide　solid　scientific　support　for　the　biological　activity　of　Lf　in　ameliorating　alcohol-induced　liver　metabolism　disorder　and　intestinal　microbiota　imbalance.