By　preparing　gold　nanoparticles-graphdiyne　(Au-GDY)　as　sensing　substrate　and　[Ru(NH3)(6)](3+)-UiO-66-NH2　MOFs　(Ru-UiO-66)　as　signaling　probe　as　well　as　linking　the　as-prepared　Au-GDY　and　Ru-UiO-66　with　the　specific　peptide　(PT)　of　MMP-7　to　form　Ru-UiO-66/PT/Au-GDY,　a　cost-effective　and　sensitive　electrochemical　sensor　was　designed　for　the　detection　of　matrix　metalloproteinase-7　(MMP-7)　which　is　an　important　biomarker　for　tumor　and　biliary　atresia.　The　superior　electrical　properties,　easy　preparation,　large　surface　area,　and　high　stability　of　Au-GDY　make　it　be　a　desirable　substrate　to　immobilize　PT.　The　unique　structure　of　UiO-66-NH2　is　beneficial　to　assemble　[Ru(NH3)(6)](3+)　probe　and　link　PT.　In　the　absence　of　MMP-7,　the　Ru-UiO-66/PT/Au-GDY　modified　electrode　exhibited　a　prominent　peak　current　of　[Ru(NH3)(6)](3+),　which　is　decreased　after　the　addition　of　target　MMP-7　since　it　can　specifically　recognize　and　cleave　PT　to　induce　the　Ru-UiO-66　leave　the　electrode.　The　experimental　results　showed　that　the　decrease　of　the　[Ru(NH3)(6)](3+)　signal　has　a　linear　relation　with　the　concentrations　of　MMP-7　in　the　range　0.01　pg　mL(-1)-10.0　ng　mL(-1).　By　optimizing　experimental　conditions,　the　achieved　detection　limit　(LOD)　for　MMP-7　is　5.0　fg　mL(-1).　The　analysis　of　real　serum　samples　has　excellent　accuracy.　The　simplicity　of　preparation　and　sensitivity　make　the　Ru-UiO-66/PT/Au-GDY　sensor　a　considerable　promising　analysis　tool　for　MMP-7　detection.