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Abstract:
IL-17 (interleukin-17) is a common proinflammatory cytokine and can be adopted as an important biomarker for several diseases; it is thus very necessary to design an effective method for monitoring IL-17. Herein, by preparing silver nanoparticles/graphdiyne (Ag/GDY) with simple electroless deposition as the sensing platform and methylene blue/UiO-66-NH2 MOFs (MB/MOFs) as the signaling-amplifier, a highly efficient sandwich-like electrochemical immunosensing (SLEI) platform was designed for IL-17 detection. The superior electrical property, easy preparation, large surface area, and high stability of Ag/GDY make it a desirable substrate to immobilize primary antibody of IL-17. Meanwhile, the unique structure of UiO-66-NH2 is beneficial to immobilize MB probe and secondary antibody. By optimizing experimental conditions, the MB current obtained at the as-designed SLEI platform increases along with IL-17 increase, and the related linearity is 1.0 pg mL(-1)-1.0 ng mL(-1) coupled with a low detection limit of 0.3 pg mL(-1). It is expected that the SLEI platform based on Ag/GDY and MB/MOFs shows some potential application for IL-17 detection.
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MICROCHIMICA ACTA
ISSN: 0026-3672
Year: 2025
Issue: 5
Volume: 192
5 . 4 0 0
JCR@2023
CAS Journal Grade:2
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ESI Highly Cited Papers on the List: 0 Unfold All
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30 Days PV: 3
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