Purpose:　This　study　aims　to　investigate　the　antibiotic　resistance　and　virulence　factors　of　Paenibacillus　campinasensis,　an　under-studied　opportunistic　pathogen,　through　whole-genome　sequencing　and　resistance　genes.　The　findings　will　contribute　to　the　development　of　effective　clinical　strategies　for　infection　control.　Patients　and　Methods:　A　1-year　and　3-month-old　boy　presenting　with　fever　and　rash　was　admitted　to　the　hospital　and　clinically　diagnosed　with　hand-foot-mouth　disease.　A　blood　culture　was　obtained,　and　the　pathogen　was　preliminarily　identified　as　belonging　to　the　genus　Paenibacillus　using　MALDI-TOF-MS.　Subsequent　16S　rDNA　PCR　product　Sanger　sequencing　confirmed　the　isolate　as　Paenibacillus　campinasensis.　Whole-genome　sequencing　and　bioinformatics　analysis　were　performed　to　evaluate　the　antibiotic　resistance　and　virulence　factors　of　the　strain.　Results:　Whole-genome　sequencing　and　bioinformatics　analysis　identified　18　resistance　genes　in　the　Paenibacillus　campinasensis　strain,　spanning　multiple　antibiotic　categories.　These　findings　suggest　the　potential　for　multidrug　resistance　in　this　bacterium,　despite　its　isolation　from　blood　without　associated　clinical　infection　in　the　patient.　Conclusion:　The　identification　of　resistance　genes　in　Paenibacillus　campinasensis　underscores　the　challenges　in　clinical　treatment　and　emphasizes　the　critical　role　of　whole-genome　analysis　in　pathogen　detection　and　characterization.　These　findings　are　vital　for　informing　effective　clinical　strategies　to　address　infections,　particularly　given　the　potential　for　antibiotic　resistance　to　hinder　treatment　outcomes,　even　in　the　absence　of　direct　disease　causation　by　the　bacterium.　©　2025　Yu　et　al.