Laser-scribed　graphene　(LSG),　with　its　high　electrical　conductivity,　tunable　surface　chemistry,　and　facile　fabrication,　has　emerged　as　a　key　material　for　developing　advanced　electrochemical　sensors.　This　review　presents　recent　advancements　in　LSG　electrodes　for　portable　and　wearable　electrochemical　biosensing,　focusing　on　both　electrode　materials　functionalization　and　their　integration　with　smartphone-based　electrochemical　sensing　systems.　It　briefly　introduces　the　design　and　working　principles　of　smartphone-based　electrochemical　sensing　system,　followed　by　an　analysis　of　the　physicochemical　properties　and　advantages　of　LSG　electrodes.　The　advances　of　LSG　electrodes　are　systematically　discussed　through　various　surface　functionalization　strategies,　including　the　integration　of　metal　nanoparticles,　polymers,　metal　complexes,　and　biomolecules.　Furthermore,　the　review　examines　the　practical　applications　of　smartphone-integrated　LSG-based　electrochemical　sensing　platforms　for　detecting　physiological　fluids　such　as　blood,　sweat,　and　saliva,　demonstrating　their　potential　for　personalized　healthcare　and　disease　management.　Finally,　the　challenges　associated　with　LSG　electrodes　in　portable　and　wearable　sensing　applications　are　addressed,　along　with　future　research　directions.　Recommendations　are　provided　to　promote　the　commercialization　and　widespread　adoption　of　this　technology.　Smartphone-integrated　LSG-based　electrochemical　sensing　platforms　is　expected　to　play　an　increasingly　crucial　role　in　the　future　of　healthcare.