Herein,　we　adapted　a　quick,　cost-effective,　and　environmentally　friendly　biological　method　for　the　synthesis　of　Ag-NPs　using　silver　nitrate　solution　as　a　precursor,　and　the　aqueous　leaf　extract　of　Lespedeza　juncea　(Chinese　lespedeza)　was　exploited　to　synthesize　Ag-NPs.　Various　physiochemical　characterization　techniques　were　used　to　characterize　the　nanoparticles.　The　UV　spectrum　revealed　a　resonance　absorption　peak　at　428　nm,　indicating　successful　synthesis　of　the　nanoparticles.　The　EDX　results　indicated　the　presence　of　Ag,　C,　O,　and　Cl　elements　in　biosynthesized　Ag-NPs　with　elemental　compositions　of　53.93%,　32.34%,　12.49%,　and　1.23%,　respectively.　The　XRD　analysis　displayed　the　crystalline　nature　of　the　nanoparticles　with　a　face-centered　cubic　lattice.　The　FTIR　spectrum　confirmed　the　involvement　of　plant-based　biological　compounds　as　reducing　and　capping　agents.　TEM　revealed　quasi-spherical　Ag-NPs　of　50　nm　or　smaller　in　size.　An　insight　into　its　biological　activities　reveals　significant　antimicrobial　activities　against　Staphylococcus　aureus　(14.1　±　0.76　mm),　Pseudomonas　aeruginosa　(11.50　±　0.40　mm),　Escherichia　coli　(16　±　0.95　mm),　and　the　fungal　strains,　viz.　Candida　albicans　(14　±　0.80　mm),　Aspergillus　flavus　(16.50　±　0.50　mm),　Aspergillus　niger　(17　±　0.86　mm)　and　afflicted　75%　mortality　to　Caenorhabditis　elegans.　Furthermore,　the　Ag-NPs　were　found　to　be　potent　inhibitors　of　the　enzymes　tyrosinase,　urease,　acetylcholinesterase,　and　butyrylcholinesterase　with　IC50　values　of　14.3　±　0.2,　19.5　±　1.1,　9.3　±　1.3,　and　32.65　±　1.9　μg/mL,　respectively.　The　overall　outcome　of　the　study　suggests　that　L.　juncea　mediated　synthesized　Ag-NPs　hold　the　potential　to　be　employed　as　a　promising　tool　for　their　antibacterial,　antifungal,　nematocidal,　and　for　a　variety　of　enzymes　inhibitory　activities.　©　2025　Wiley　Periodicals　LLC.