Spider　silk　is　a　natural　biomaterial　that　has　attracted　considerable　attention　because　of　its　exceptional　physical　and　chemical　properties.　Developing　fiber　materials　that　combine　high　strength　and　toughness　remains　a　significant　challenge,　as　increasing　fiber　strength　often　reduces　toughness.　However,　spider　silk　achieves　an　optimal　balance　of　these　properties　through　gelation　and　stretching　of　its　spinning　solution.　This　remarkable　feature　has　inspired　extensive　efforts　to　produce　artificial　fibers　that　mimic　natural　spider　silk’s　materials,　structures,　and　spinning　mechanisms.　For　decades,　researchers　have　focused　on　artificially　spun　recombinant　spidroins　to　replicate　the　extraordinary　mechanical　properties　of　natural　spider　silk.　This　paper　investigates　the　relationship　between　the　structure　and　function　of　spidroins.　It　reviews　the　primary　methods　for　synthesizing　spider　silk　fibers,　emphasizing　their　advantages　and　limitations.　These　insights　provide　a　theoretical　foundation　for　the　design　of　artificial　protein　fibers　and　spinning　equipment.　This　study　addresses　the　challenges　and　unresolved　issues　in　the　current　research.　It　proposes　future　directions,　advancing　our　understanding　of　spidroins　fiber　production　and　establishing　a　foundation　for　further　studies.　©　2025　Informa　UK　Limited,　trading　as　Taylor　&　Francis　Group.