With　increasing　public　awareness　of　healthcare,　the　demand　for　early　screening　of　cancer　biomarkers　has　grown　substantially.　Nanozymes,　with　their　unique　catalytic　and　sensing　properties,　have　emerged　as　promising　alternatives　in　this　field.　Herein,　a　cascade　catalytic　system　was　developed　by　integrating　natural　glucose　oxidase　with　oxygen　vacancy-rich　trimetallic　oxide　nanozymes.　Through　specific　immunorecognition,　efficient　conversion　of　biological　signals　into　measurable　outputs　was　achieved,　enabling　the　construction　of　a　dual-mode　colorimetric　and　photothermal　sensing　platform　for　the　sensitive　detection　of　carcinoembryonic　antigens.　Density　functional　theory　calculations　revealed　that　the　trimetallic　synergistic　strategy　effectively　modulates　the　distribution　of　active　sites　and　electronic　structures.　The　presence　of　abundant　oxygen　vacancies　and　a　multilayered　stacked　morphology　further　enhanced　the　peroxidase-like　activity　and　substrate　activation　capability.　Under　optimized　conditions,　the　photothermal　mode　exhibited　a　broad　linear　range　from　0.05　to　50　ng　mL-1,　while　the　colorimetric　mode　showed　a　sensitive　response　from　0.015　to　100　ng　mL-1,　with　corresponding　detection　limits　of　14.2　and　9.7　pg　mL-1,　respectively.　This　work　demonstrates　that　the　synergy　between　multimetallic　components　and　oxygen　vacancies　can　significantly　enhance　artificial　enzyme　performance,　offering　a　robust　sensing　platform　with　dual-signal　output.　The　strategy　not　only　shows　excellent　applicability　in　real　sample　analysis　but　also　provides　a　new　avenue　for　high-throughput　and　multidimensional　bioanalytical　applications.