With　the　expanding　insights　into　the　＂gut-brain　axis,＂　the　association　between　neurodevelopmental　disorders　(NDDs)　and　gut　microbiota　has　gained　extensive　attention　in　scientific　research.　Maternal　immune　activation　(MIA)　in　pregnant　females　is　a　crucial　environmental　risk　factor　for　NDDs　in　offspring.　Polyriboinosinic-polyribocytidylic　acid　(Poly　I:C)　belongs　to　a　class　of　synthetic　analogs　of　double-stranded　RNA　used　to　induce　MIA　in　rodents　and　is　widely　used　in　scientific　research.　The　dataset　presents　16S　rRNA　sequencing　data　of　fecal　microbiota　from　juvenile　female　rats　following　prenatal　Poly　I:C　exposure.　It　will　help　to　clarify　the　impact　of　prenatal　Poly　I:C　exposure　on　the　intestinal　microbiota　char-acteristics　of　juvenile　female　offspring　rats,　reveal　the　role　of　the　intestinal　microbiota　in　the　pathophysiological　changes　related　to　maternal　immune　activation　and　provide　new　in-sights　into　the　mechanism　of　the　microbiota-gut-brain　axis　in　neurodevelopmental　disorders.　(c)　2025　Published　by　Elsevier　Inc.　This　is　an　open　access　article　under　the　CC　BY　license　(http://creativecommons.org/licenses/by/4.0/)