Alkaline　phosphatase　(ALP)　is　a　vital　protein　catalyst　that　holds　a　central　role　in　modulating　protein　metabolism　and　function　throughout　organisms,　and　serves　as　a　reliable　foundation　for　the　treatment　of　human　diseases.　Thus,　the　development　of　a　sensitive　and　selective　detection　strategy　for　ALP　is　considerable　scientific　and　practical　significance.　In　this　study,　SiO2　was　chosen　as　the　carrier　for　encapsulating　Ru-1　luminophores　to　effectively　prevent　the　leakage　of　Ru-1　molecules.　Meanwhile,　during　the　preparation　process,　the　introduction　of　a　poor　solvent　induces　aggregation　of　Ru-1　molecules　into　nanoparticles,　thereby　enhancing　emission　through　the　aggregation-induced　emission　effect.　Furthermore,　using　an　in-situ　growth　method,we　developed　a　phosphorescence-based　detection　strategy,　which　builds　on　Ru-SiO2-MnO2　nanocomposites.　L-ascorbic　acid　(AA)　can　be　generated　by　ALP,　which　catalyzes　the　hydrolysis　of　sodium　L-ascorbyl-2-phosphate　(AAP).　This　process　further　breaks　down　MnO2　and　triggers　the　phosphorescence　sensor　through　a　＂turn-on＂　mechanism.　Moreover,　by　exploiting　the　capacity　of　Na3VO4　to　suppress　ALP　activity,　this　phosphorescent　method　was　further　employed　for　the　identification　of　ALP　inhibitors.　Under　the　optimized　experimental　conditions,　the　phosphorescent　intensity　displayed　a　linear　association　with　ALP　concentrations　ranging　from　0.05　to　17　U/L,　while　the　limit　of　detection　was　as　low　as　0.031　U/L.　The　proposed　strategy　eliminates　the　need　for　complex　washing　or　modification　procedures　and　avoids　the　aggregation-caused　quenching　phenomenon　of　traditional　organic　dyes,　thus　standing　out　as　an　uncomplicated,　economical　＂turn-on＂　phosphorescent　detection　method　for　ALP.　This　approach　also　demonstrated　excellent　applicability　in　the　analysis　of　serum　samples.