Cutaneous　squamous　cell　carcinoma　(cSCC),　the　second　most　common　skin　cancer,　requires　early　and　accurate　detection　to　optimize　clinical　management.　Fluorescence　imaging　has　emerged　as　a　non-invasive　and　cost-effective　tool　with　high　sensitivity　for　tumor　diagnosis;　however,　existing　probes　for　cSCC　are　limited　in　achieving　rapid　and　specific　imaging.　In　this　study,　we　introduce　FC-1,　a　dual-tandem　activatable　fluorescent　probe　designed　for　precise　cSCC　diagnosis　by　simultaneously　targeting　fibroblast　activation　protein　alpha　and　cathepsin　C　(CTSC).　This　dual-locked　activation　strategy　effectively　reduces　nonspecific　signals　in　normal　tissues　while　enhancing　diagnostic　specificity.　In　vivo　studies　in　SCC-7　xenografted　mice　demonstrated　FC-1＇s　ability　to　visualize　subcutaneous　tumors　with　high　sensitivity　and　specificity.　Moreover,　FC-1　enabled　clear　differentiation　between　cSCC　and　keloids.　The　probe＇s　dual-activation　mechanism　and　robust　performance　underscore　its　potential　as　a　clinically　translatable　tool　for　early　cSCC　detection　and　differential　diagnosis.