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author:

Zeng, Zhiping (Zeng, Zhiping.) [1] (Scholars:曾志平) | Ma, Jing (Ma, Jing.) [2] (Scholars:马靖) | Chen, Xuanze (Chen, Xuanze.) [3] | Xu, Canhua (Xu, Canhua.) [4] (Scholars:许灿华)

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Scopus SCIE

Abstract:

In this paper, we propose a promising super-resolution imaging scheme in fluorescence lifetime domain (lifetime super-resolution optical fluctuation imaging, ltSOFI). ltSOFI has the potential to obtain super-resolution images by taking advantage of fluorescence lifetime blinking under wide-field lifetime detection. The proof-of-concept for ltSOFI was demonstrated through numerical simulation of high-order cumulant analysis on fluorescence lifetime blinking emitters. As a tentative experimental demonstration, we obtained super-resolution lifetime imaging from time-lapse FLIM recording of HeLa cells expressing a cAMP sensor using ltSOFI method. ltSOFI is expected to initiate a new dimension in the lifetime domain for blinking-based super-resolution microscopy.

Keyword:

fluorescence fluctuation lifetime blinking super-resolution

Community:

  • [ 1 ] [Zeng, Zhiping]Fuzhou Univ, Coll Phys & Informat Engn, 2 North Wulongjiang Rd, Fuzhou 350108, Fujian, Peoples R China
  • [ 2 ] [Ma, Jing]Fuzhou Univ, Coll Phys & Informat Engn, 2 North Wulongjiang Rd, Fuzhou 350108, Fujian, Peoples R China
  • [ 3 ] [Xu, Canhua]Fuzhou Univ, Coll Phys & Informat Engn, 2 North Wulongjiang Rd, Fuzhou 350108, Fujian, Peoples R China
  • [ 4 ] [Chen, Xuanze]Cowin Venture, 1468 West Nanjing Rd, Shanghai 200040, Peoples R China

Reprint 's Address:

  • 许灿华

    [Xu, Canhua]Fuzhou Univ, Coll Phys & Informat Engn, 2 North Wulongjiang Rd, Fuzhou 350108, Fujian, Peoples R China;;[Chen, Xuanze]Cowin Venture, 1468 West Nanjing Rd, Shanghai 200040, Peoples R China

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Source :

JOURNAL OF MICROSCOPY

ISSN: 0022-2720

Year: 2019

Issue: 2

Volume: 274

Page: 87-91

1 . 5 7 5

JCR@2019

1 . 5 0 0

JCR@2023

ESI Discipline: BIOLOGY & BIOCHEMISTRY;

ESI HC Threshold:189

JCR Journal Grade:3

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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