A　silk　ftbroin/collagen/hyaluronic　acid　(SF/COL/HA)　composite　scaffold　was　prepared　via　admixing,　cross-linking,　and　lyophilizing　processes.　We　studied　its　physicochemical　and　biological　properties,　such　as　water　absorption,　porosity,　weight　loss,　and　biocompatibility.　The　optimal　ratio　of　SF/COL/HA　scaffold　was　3:6.5:0.5.　Then,　the　optimal　ratio　of　scaffold　incorporating　pilose　antler　polypeptides　(PAPs)-PLGA　microspheres　was　prepared,　and　their　compatibility　was　studied.　PAP-SF/COL/HA　scaffold　had　favorable　adhesion　and　proliferation.　A　rabbit　cartilage　defect　model　was　established.　The　repair　effect　of　cartilage　defects　was　observed　and　evaluated　among　PAP-SF/COL/HA,　SF/COL/HA,　and　sham　operation　groups.　The　defects　were　almost　completely　repaired　after　13　weeks　in　the　PAP-SF/COL/HA　group,　thereby　indicating　that　the　PAP-SF/COL/HA　composite　had　a　favorable　effect　on　articular　cartilage　repair.