This　work　described　a　sensitive　thrombin　analysis　method　employing　metal-waveguide-capillary　(MWC)　photometry　with　a　sandwich　aptamer-gold　nanorods　(GNRs)　as　a　signal　transporting　probe.　The　sandwich　aptasensor　consisted　of　a　pair　of　thrombin-binding　aptamers:　one　29-mer　aptamer　modified　with　magnetic　beads　as　capture　aptamers;　and　one　15-mer　aptamer　binding　with　GNRs　as　report　aptamers.　Through　the　measurement　of　GNRs　from　the　sandwich　complex,　the　MWC　photometry　enabled　to　detect　thrombin　with　a　linear　range　from　0.068　nM　to　1.09　nM,　and　with　a　detection　limit　as　low　as　0.02　nM.　The　detection　limit　was　improved　68-fold　vs.　that　of　commercial　spectrophotometer　with　1　cm　cuvette.　(C)　2016　Elsevier　B.V.　All　rights　reserved.