Magnetic graphene oxide-based electrochemiluminescent aptasensor for thrombin - Details

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EI Scopus SCIE

Abstract：

A　new　electrochemiluminescence　(ECL)　reagent,　iridium(III)　complex　(Kill)　complex),　has　been　synthesized　and　applied　to　develop　an　ECL　biosensor　for　thrombin.　The　Ir(III)　complex　has　been　conjugated　to　aptamers　firstly　and　adsorbed　on　magneto-controlled　magnetic　graphene　oxide.　In　the　presence　of　the　target　(thrombin),　the　aptamers　will　release　into　solution　and　result　in　increased　ECL　intensity.　The　enhanced　ECL　intensity　has　a　direct　relationship　with　the　logarithm　of　thrombin　concentration　in　the　range　of　2.0　x　10(-9)　to　5.0　x　10(-8)　mol/L.　The　detection　limit　was　1.3　x　10(-9)　mol/L　(S/N　=　3).　The　proposed　method　has　good　stability　and　reproducibility.　(C)　2012　Elsevier　Ltd.　All　rights　reserved.

Keyword：

Aptamer Electrochemiluminescence (ECL) Iridium(III) complex Magnetic graphene oxide Thrombin

Community：

[ 1 ] [Jin, Guixiao]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
[ 2 ] [Lu, Lijun]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
[ 3 ] [Gao, Xiaoyao]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
[ 4 ] [Li, Mei-Jin]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
[ 5 ] [Qiu, Bin]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
[ 6 ] [Lin, Zhenyu]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
[ 7 ] [Yang, Huanghao]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
[ 8 ] [Chen, Guonan]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China

Reprint 's Address：

李梅金
[Li, Mei-Jin]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China

Email：

mjli@fzu.edu.cn |
zylin@fzu.edu.cn

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