A　supercooled　disk　electrode　is　fabricated,　around　which　the　temperature　of　aqueous　solution　is　below　its　normal　freezing　point.　The　supercooled　electrode　demonstrates　thermal　stability　and　its　surface　temperature　can　be　adjusted　by　changing　an　additional　heating　current.　Theoretical　simulation　shows　that　the　supercooled　region　is　restricted　within　a　small　volume　around　the　disk　electrode.　The　electrochemical　behavior　of　cytochrome　c　was　investigated　at　supercooled　electrode　for　the　first　time.　(C)　2014　Elsevier　B.V.　All　rights　reserved.