A　new　flow-through　electrochemical　aptasensor　was　designed　for　ultrasensitive　monitoring　of　adenosine　triphosphate　(ATP)　by　coupling　microvalve-programmable　capillary　column　with　CdS-functionalized　DNA　concatamer　for　signal　amplification.　Initially,　a　layer　of　primary　DNA-conjugated　polyacrylamide　hydrogel　was　covalently　linked　onto　the　internal　surface　of　capillary　column,　and　then　an　automated　sequenctial　injection　format　with　a　syringe　pump　was　employed　for　development　of　the　programmable　capillary-based　aptasensor.　In　the　presence　of　target　DNA　aptamer,　the　immobilized　primary　DNA　hybridized　with　partial　bases　of　the　aptamer.　The　excess　aptamer　fregment　could　trigger　the　formation　of　DNA　concatamer　between　auxiliary　DNA(1)　and　CdS-labeled　auxiliary　DNA(2).　Upon　target　ATP　introduction,　a　specific　ATP-aptamer　reaction　was　excuated,　thereby　resulting　in　the　release　of　CdS-functionalized　DNA　concatamer　from　the　capillary.　Subsenquent　anodic　stripping　voltammetric　detection　of　cadmium　released　under　acidic　conditions　from　the　released　CdS　nanoparticles　could　be　conducted　in　a　homemade　detection　cell.　Under　optimal　conditions,　the　dynamic　concentration　range　spanned　from　0.1　pM　to　10　nM　ATP　with　a　detection　limit　of　0.06　pM　ATP.　The　electrochemical　aptasensor　showed　good　reproducibility,　selectivity,　and　stability.　In　addition,　the　methodology　was　evaluated　for　the　analysis　of　ATP　spiked　serum　samples,　and　the　recoveries　was　81-140%.　(C)　2013　Elsevier　B.V.　All　rights　reserved.