Direct detection of circulating microRNAs in serum of cancer patients by coupling protein-facilitated specific enrichment and rolling circle amplification - Details

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Abstract：

We　have　developed　a　simple　method　for　direct　detection　of　circulating　microRNAs　in　serum　by　using　the　p19　protein-functionalized　magnetic　beads　(PFMBs)　for　specific　enrichment　and　rolling　circle　amplification　(RCA).　The　detection　limit　for　microRNAs　is　1　fM.　Therefore,　the　proposed　method　has　the　potential　of　being　used　in　the　analysis　of　circulating　microRNAs　and　clinical　diagnosis.

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[ 1 ] [Hong, Cheng-Yi]Fuzhou Univ, Coll Chem & Chem Engn, Key Lab Anal & Detect Food Safety MOE & Fujian Pr, Fuzhou 350108, Peoples R China
[ 2 ] [Chen, Xian]Fuzhou Univ, Coll Chem & Chem Engn, Key Lab Anal & Detect Food Safety MOE & Fujian Pr, Fuzhou 350108, Peoples R China
[ 3 ] [Li, Juan]Fuzhou Univ, Coll Chem & Chem Engn, Key Lab Anal & Detect Food Safety MOE & Fujian Pr, Fuzhou 350108, Peoples R China
[ 4 ] [Chen, Guonan]Fuzhou Univ, Coll Chem & Chem Engn, Key Lab Anal & Detect Food Safety MOE & Fujian Pr, Fuzhou 350108, Peoples R China
[ 5 ] [Yang, Huang-Hao]Fuzhou Univ, Coll Chem & Chem Engn, Key Lab Anal & Detect Food Safety MOE & Fujian Pr, Fuzhou 350108, Peoples R China
[ 6 ] [Chen, Jing-Hua]Fujian Med Univ, Fac Pharm, Dept Pharmaceut Anal, Fuzhou 350004, Peoples R China

Reprint 's Address：

[Chen, Jing-Hua]Fujian Med Univ, Fac Pharm, Dept Pharmaceut Anal, Fuzhou 350004, Peoples R China

Email：

cjh_huaxue@126.com |
hhyang@fio.org.cn

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Direct detection of circulating microRNAs in serum of cancer patients by coupling protein-facilitated specific enrichment and rolling circle amplification
2014，Chemical Communications

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CHEMICAL COMMUNICATIONS

ISSN： 1359-7345

Year： 2014

Issue： 25

Volume： 50

Page： 3292-3295

6 . 8 3 4

JCR@2014

4 . 3 0 0

JCR@2023

ESI Discipline： CHEMISTRY;

ESI HC Threshold：268

JCR Journal Grade：1

CAS Journal Grade：2

Cited Count：

WoS CC Cited Count： 41

SCOPUS Cited Count： 41

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 0

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化学学院本学院/部未明确归属的数据

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