A　novel　polymeric　monolith　with　hydrophilic　interaction　and　strong　anion-exchange　mixed-mode　has　been　fabricated　for　pressurized　capillary　electrochromatography　by　an　in　situ　copolymerization　of　vinylbenzyl　trimethylammonium　chloride　(VBTA)　and　bisphenol　A　glycerolate　dimethacrylate　(BisGMA).　The　optimization　of　the　polymerization　mixture　composition　has　been　investigated,　and　column　characteristics　in　terms　of　mechanical　stability,　permeability　and　reproducibility　have　been　studied　in　detail.　Linear　responses　between　back　pressure　and　flow　rate　have　been　achieved　in　different　solvents.　The　absolute　value　of　swelling　propensity　(SP)　factor　for　poly(VBTA-co-BisGMA)　monolith　is　0.41,　and　the　degree　of　permeability　drop　from　pure　ACN　to　water　is　about　45%.　An　acceptable　mechanical　stability　of　the　column　is　obtained.　The　suitable　reproducibility　is　also　measured　with　the　RSD　for　day-to-day　(n　=　3)　of　retention　time　and　column　efficiency　less　than　3.3%,　and　the　RSD　for　batch-to-batch　(n　=　3)　less　than　5.3%,　respectively.　Under　the　optimum　conditions,　the　mixed-mode　of　hydrophilic　interaction　and　strong　anion-exchange　has　been　carried　out,　and　efficient　electrochromatography　profiling　of　various　polar　compounds　including　neutral　phenols,　negatively　charged　benzoic　acids　and　positively　charged　nucleic　acid　bases　and　nucleosides　are　achieved,　respectively.　(C)　2013　Elsevier　B.V.　All　rights　reserved.