A　novel　monolithic　stationary　phase　with　mixed　mode　of　hydrophilic　and　strong　anion　exchange　(SAX)　interactions　based　on　in　situ　copolymerization　of　pentaerythritol　triacrylate　(PETA),　N,N-dimethyl-N-methacryloxyethyl　N-(3-sulfopropyl)　ammonium　betaine　(DMMSA)　and　a　selected　quaternary　amine　acrylic　monomer　was　designed　as　a　multifunctional　separation　column　for　CEC.　Although　the　zwitterionic　functionalities　of　DMMSA　and　hydroxy　groups　of　PETA　on　the　surface　of　the　monolithic　stationary　phase　functioned　as　the　hydrophilic　interaction　(HI)　sites,　the　quaternary　amine　acrylic　monomer　was　introduced　to　control　the　magnitude　of　the　EOF　and　provide　the　SAX　sites　at　the　same　time.　Three　different　quaternary　amine　acrylic　monomers　were　tested　to　achieve　maximum　EOF　velocity　and　highest　plate　count.　The　fabrication　of　the　zwitterionic　monolith　(designated　as　HI　and　SAX　stationary　phase)　was　carried　out　when　[2-(acryloyloxy)　ethyl]　trimethylammonium　methylsulfate　was　used　as　the　quaternary　amine　acrylic　monomer.　The　separation　mechanism　of　the　monolithic　column　was　discussed　in　detail.　For　charged　analytes,　a　mixed　mode　of　HI　and　SAX　was　observed　by　studying　the　influence　of　mobile　phase　pH　and　salt　concentration　on　their　retentions　on　the　poly(PETA-co-　DMMSA-co-[2-(acryloyloxy)　ethyl]　trimethylammonium　methylsulfate)　monolithic　column.　The　optimized　monolith　showed　good　separation　performance　for　a　range　of　polar　analytes　including　nucleotides,　nucleic　acid　bases　and　nucleosides,　phenols,　estrogens　and　small　peptides.　The　column　efficiencies　greater　than　192　000　theoretical　plates/m　for　estriol　and　135　000　theoretical　plates/m　for　charged　cytidine　were　obtained.