A　novel　multiplexed　stripping　voltammetric　immunoassay　protocol　was　designed　for　the　simultaneous　detection　of　multiple　biomarkers　(CA　125,　CA　15-3,　and　CA　19-9　used　as　models)　using　PAMAM　dendrimer-metal　sulfide　quantum　dot　(QD)　nanolabels　as　distinguishable　signal　tags　and　trifunctionalized　magnetic　beads　as　an　immunosensing　probe.　The　probe　was　prepared　by　means　of　co-immobilization　of　primary　monoclonal　anti-CA　125,　anti-CA　15-3　and　anti-CA　19-9　antibodies　on　a　single　magnetic　bead.　The　PAMAM　dendrimer-metal　sulfide　QD　nanolabels　containing　CdS,　ZnS　and　PbS　were　synthesized　by　using　in　situ　synthesis　method,　which　were　utilized　for　the　labeling　of　polyclonal　rabbit　anti-CA　125,　anti-CA　15-3　and　anti-CA　19-9　detection　antibodies,　respectively.　A　sandwich-type　immunoassay　format　was　adopted　for　the　simultaneous　determination　of　target　biomarkers　in　a　low-binding　microtiter　plate.　The　subsequent　anodic　stripping　voltammetric　analysis　of　cadmium,　zinc,　and　lead　components　released　by　acid　from　the　corresponding　QD　nanolabels　was　conducted　at　an　in　situ　prepared　mercury　film　electrode　based　on　the　difference　of　peak　potentials.　Experimental　results　indicated　that　the　multiplexed　immunoassay　enabled　the　simultaneous　detection　of　three　cancer　biomarkers　in　a　single　run　with　wide　dynamic　ranges　of　0.01-50　U　mL(-1)　and　detection　limits　(LODs)　of　0.005　U　mL(-1).　Intra-assay　and　inter-assay　coefficients　of　variation　(CVs)　were　less　than　7.2%　and　10.4%,　respectively.　No　significant　differences　at　the　0.05　significance　level　were　encountered　in　the　analysis　of　10　clinical　serum　specimens　between　the　multiplexed　immunoassay　and　a　commercially　available　enzyme-linked　immunosorbent　assay　(ELISA).　(C)　2013　Elsevier　B.V.　All　rights　reserved.