Four　new　aculeatusquinones　A-D　(1-4)　and　five　known　compounds,　(5aS,6S,7S)-3,7-dihydroxy-6-methoxy-1,4,6,9-tetramethyl-6,7-dihydro-5aH-dibenzo[b,e][1,4]dioxepine-8,11-dione　(5),　3,8-dihydroxy-1,4,6,9-tetramethyldibenzo[b,e][1,4]dioxepin-11-one　(6),　4-O-demethylbarbatic　acid　(7),　atraric　acid　(8),　and　2,5-dimethyl-1,3-benzenediol　(9),　were　isolated　from　the　marine-derived　fungus　Aspergillus　aculeatus.　The　structures　of　the　new　compounds　were　elucidated　by　spectroscopic　methods,　including　one-　and　two-dimensional　NMR　and　high-resolution　mass　spectrometric　analyses.　Two　new　compounds　(2　and　4)　showed　cytotoxic　effects　on　the　HL-60,　K562,　and　A-549　cell　lines,　with　IC50　values　ranging　from　5.4　mu　M　to　76.1　mu　M.